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  • Title: Processing of photoaptamer microarrays.
    Author: Petach H, Ostroff R, Greef C, Husar GM.
    Journal: Methods Mol Biol; 2004; 264():101-10. PubMed ID: 15020783.
    Abstract:
    Photoaptamers are single-stranded nucleic acids selected for their high affinity to specific proteins of interest. Photoaptamer microarrays capture and quantify proteins from complex samples using a unique protocol that leverages both high-affinity capture with covalent retention of analytes. The initial capture of proteins from solution is similar to the well-known antibody capture, but the "secondary binding event" affected by photoaptamers is a covalent crosslink between the photoaptamer capture agent and the protein analyte. The nature of this specific covalent reaction allows a unique microarray processing that is described in detail in this chapter.
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