These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Genotyping of hepatitis C virus by melting curve analysis with SYBR Green I.
    Author: Fujigaki H, Takemura M, Takahashi K, Yamada Y, Fujii H, Wada H, Saito K, Ohnishi H, Seishima M.
    Journal: Ann Clin Biochem; 2004 Mar; 41(Pt 2):130-2. PubMed ID: 15025803.
    Abstract:
    BACKGROUND: Recent studies have focused on whether different hepatitis C virus (HCV) genotypes are associated with different profiles of pathogenicity, infectivity, and response to antiviral therapy. We needed to develop a convenient screening test for HCV genotypes 1 and 2. METHOD: We tested 55 patients with known chronic HCV infection. Viral RNA was extracted from serum samples using an automatic viral RNA purification system, and HCV genotypes were determined by reverse transcriptase-polymerase chain reaction using LightCycler melting curve analysis with SYBR Green I. RESULTS: HCV RNA was detected in all samples and each genotype was determined. The mean (standard deviation) melting temperatures for subtypes 1b (n = 32), 2a (n = 15) and 2b (n = 8) were 93.14 degrees C (0.51 degrees C), 91.08 degrees C (0.49 degrees C) and 91.77 degrees C (0.27 degrees C), respectively. Genotypes 1 and 2 were differentiated within 3 h by this method. CONCLUSIONS: Our melting curve analysis is a rapid and convenient screening test for differential identification of HCV genotypes 1 and 2.
    [Abstract] [Full Text] [Related] [New Search]