These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Participation of the matrix metalloproteinase inhibitor in Thy-1 nephritis.
    Author: Mitani O, Katoh M, Shigematsu H.
    Journal: Pathol Int; 2004 Apr; 54(4):241-50. PubMed ID: 15028025.
    Abstract:
    What influence would be shown in Thy-1 glomerulonephritis when the synthetic matrix metalloproteinase (MMP) inhibitor SI-27 is administered? Five groups of 80 male Wistar rats were studied: healthy group; treated healthy group; nephritic group; pretreated nephritic group; and post-treated nephritic group. SI-27 treatment of nephritic animals was initiated either 2 days before or 2 days after anti-Thy-1.1 antibody injection. On days 7, 14, 26 and 42 after disease induction, we examined renal histology, extracellular matrix (ECM) constituent, and MMP activity. SI-27 treated Thy-1 groups resulted in significant reduction of glomerular cells including alpha-smooth muscle actin (alpha-SMA) positive mesangial cells and suppressed expression of type IV collagen at 7 days. Moreover, type I collagen was also decreased by SI-27 at 42 days. However, glomerular cell numbers did not show any significant changes at 14, 26 and 42 days. In gelatin zymography, the gelatinolytic band for MMP-9 was expressed in SI-27 treated Thy-1 nephritis groups, although it was not expressed in the nephritic group at day 7. However, the expression of MMP-9 was no longer seen at 14, 26 and 42 days. The bands for an active form of MMP-2 were expressed throughout the experimental period in the Thy-1 nephritic groups. These results suggest that MMP plays an important role in the development of Thy-1 nephritis, and even if the synthetic MMP inhibitor intercepts the initial increase of glomerular cells and matrices, it does not inhibit recovery to normal glomerular capillary structures in Thy-1 nephritis.
    [Abstract] [Full Text] [Related] [New Search]