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  • Title: Sequence-specific assignments of methyl groups in high-molecular weight proteins.
    Author: Yang D, Zheng Y, Liu D, Wyss DF.
    Journal: J Am Chem Soc; 2004 Mar 31; 126(12):3710-1. PubMed ID: 15038713.
    Abstract:
    A new 3D multiple-quantum (H)CCmHm-TOCSY experiment is proposed to assign methyl resonances in high-molecular weight proteins, on the basis of spectral patterns and prior backbone assignments. The favorable relaxation properties of the multiple-quantum coherences and the slow decays of in-phase methyl 13C magnetizations optimize performance of the proposed experiment for application to large proteins. The experiment has been demonstrated on an acyl carrier protein synthase (trimer, 42 kDa, overall correlation time of 26 ns) at 25 degrees C, and 63 out of 67 nonmethionine methyl groups have been assigned.
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