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  • Title: [Screening and identification of human telomerase reverse transcriptase epitopes from random phage display peptide library].
    Author: Miao PH, Liu BY, Zheng SG, Pang JX, Xu JP.
    Journal: Di Yi Jun Yi Da Xue Xue Bao; 2004 Mar; 24(3):252-5. PubMed ID: 15041534.
    Abstract:
    OBJECTIVE: To identify and characterize the epitopes of human telomerase reverse transcriptase (hTERT). METHODS: A random phage displayed dodecapeptide library was screened with anti-hTERT antibody. The selected phage clones were identified by sandwich enzyme-linked immunosorbent assay, anti-hTERT antibody blocking assay and competitive inhibition assay. RESULTS: After 3 rounds of screening, 13 of 24 randomly selected phage clones were identified as positive clones that could specifically bind to anti-hTERT antibody but not to normal mouse IgG. Amino acid sequences deduced from DNA sequences showed 11 different sequences with high percentage of histone and hydrophilic amino acids. CONCLUSION: Eleven epitopes have been obtained that can effectively mimic hTERT, which will be useful for the research of small-molecule inhibitor targeting at hTERT.
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