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  • Title: Threonine is the best substrate for D-lactate formation in octopus tentacle.
    Author: Akagi S, Ohmori S.
    Journal: Amino Acids; 2004 Mar; 26(2):169-74. PubMed ID: 15042447.
    Abstract:
    Carbon sources for D-lactate and enzyme activities related to D-lactate formation were investigated using cell-free homogenates of Octopus vulgaris tentacle tissue. The results are as follows: a) The best precursor for D-lactate formation was threonine and second best precursors were glycine and fructose-1,6-bisphosphate. Threonine and glycine served as precursors only in presence of glutathione. b) Both amino acids were precursors for methylglyoxal from which D-lactate was synthesized. Alanine, cysteine and serine were not precursors. We present a metabolic map for D-lactate formation in octopus in order to explain these experimental results.
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