These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effect of cold-ischemia time on nuclear factor-kappaB activation and inflammatory response in graft after orthotopic liver transplantation in rats.
    Author: Gu XP, Jiang Y, Xu FT, Qiu YD, Ding YT.
    Journal: World J Gastroenterol; 2004 Apr 01; 10(7):1000-4. PubMed ID: 15052682.
    Abstract:
    AIM: To study the mechanism and effect of nuclear factor-kappaB (NF-kappaB) activation and inflammatory response on the extended cold-preserved graft injury after orthotopic liver transplantation (OLT). METHODS: OLT was performed in rats with varying time of cold ischemia grafts (6, 18 and 24 h in University Wisconsin solution at 4 degrees ). We determined the time of NF-kappaB activation and expression of tumor necrosis factor-alpha (TNF-alpha), cytokine-inducible neutrophil chemoattractant (CINC), and intercellular adhesion molecule-1 (ICAM-1) within 6 h after reperfusion. Serum alarming aminotransferase (ALT), neutrophil sequestration, circulating neutrophil CD11b and L- selectin expression were also evaluated. RESULTS: The accumulation of neutrophils in the graft was significantly increased in the 18 h and 24 h cold-ischemia groups within 0.5 h after reperfusion, compared with the 6 h group. But the strongly activated neutrophils was slightly increased at 2 h after reperfusion and remained at high levels 4 h after reperfusion, which was synchronized with the common situation of recipients after transplantation. Prolonged cold-preservation did not affect neutrophil accumulation and activation. NF-kappaB activation preceded the expression of TNF-alpha, CINC, and ICAM-1 in the liver, which was significantly increased with prolonged cold preservation. In prolonged cold preserved grafts, prominently elevated NF-kappaB activation occurred at 0.5 h and 1 h, compared with that at 2 h after reperfusion, which was consistent with greatly increased intrahepatic TNF-alpha response. CONCLUSION: NF-kappaB activation is correlated with the expression of TNF-alpha, CINC, and ICAM-1 in vivo in OLT rats. Extended cold preservation of grafts might up-regulate TNF-alpha, CINC, and ICAM-1 expression in the grafts, most probably through elevated NF-kappaB activation, and might contribute to neutrophil infiltration in the grafts after reperfusion. Elevated NF-kappaB activity is harmful to inflammatory response in the grafts, and inhibited NF-kappaB activity might protect against early graft injury after liver transplantation.
    [Abstract] [Full Text] [Related] [New Search]