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  • Title: Direct detection of genomic DNA by enzymatically amplified SPR imaging measurements of RNA microarrays.
    Author: Goodrich TT, Lee HJ, Corn RM.
    Journal: J Am Chem Soc; 2004 Apr 07; 126(13):4086-7. PubMed ID: 15053580.
    Abstract:
    A novel surface enzymatic reaction scheme that amplifies the optical response of RNA microarrays to the binding of complementary DNA is developed for the direct detection and analysis of genomic DNA. The enzyme RNase H is shown to selectively and repeatedly destroy RNA from DNA-RNA heteroduplexes on gold surfaces; when used in conjunction with the label-free technique of surface plasmon resonance (SPR) imaging, DNA oligonucleotides can be detected at a concentration of 1 fM. This enzymatically amplified SPR imaging methodology is then utilized to detect and identify the presence of the TSPY gene in human genomic DNA without PCR amplification.
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