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  • Title: Detection of cytotoxic necrotizing factor types 1 and 2 among fecal Escherichia coli isolates from Brazilian children with and without diarrhea.
    Author: Tavechio AT, Marques LR, Abe CM, Gomes TA.
    Journal: Mem Inst Oswaldo Cruz; 2004 Feb; 99(1):81-3. PubMed ID: 15057352.
    Abstract:
    The enteropathogenic role of cytotoxic necrotizing factor (CNF)-producing Escherichia coli was investigated by searching cnf genes among 2074 isolates from 200 children with and 200 without acute diarrhea in Brazil. Fourteen (7%) cases versus 10 (5%) control children carried at least one cnf positive isolate (P = 0.50) and most isolates expressed CNF type 1. DNA sequences of virulence factors of extraintestinal pathogenic E. coli (ExPEC) were detected in 78.6% of CNF1-producing isolates. Besides not being associated with human acute diarrhea, the CNF1-producing isolates here identified may represent potential ExPEC transitorily composing the normal intestinal flora.
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