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  • Title: Isolation and characterization of site-specific DNA-methyltransferases from Bacillus coagulans K.
    Author: Svadbina IV, Zelinskaya NV, Kovalevskaya NP, Zheleznaya LA, Matvienko NI.
    Journal: Biochemistry (Mosc); 2004 Mar; 69(3):299-305. PubMed ID: 15061697.
    Abstract:
    Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5'-CTCTTC-3'/5'-GAAGAG-3' from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5'-CTCTTC-3'.
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