These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: beta-Naphthoflavone disrupts cortisol production and liver glucocorticoid responsiveness in rainbow trout.
    Author: Aluru N, Vijayan MM.
    Journal: Aquat Toxicol; 2004 Apr 28; 67(3):273-85. PubMed ID: 15063076.
    Abstract:
    We investigated the impact of aryl hydrocarbon receptor (AhR) activation on cortisol production in rainbow trout (Oncorhynchus mykiss) using beta-naphthoflavone (BNF) and alpha-naphthoflavone (ANF) as agonist and antagonist of AhR, respectively. Fish were given a single intraperitoneal injection of either corn oil alone or corn oil containing ANF (50 mg/kg body mass), BNF (50 mg/kg) or a combination of both (ANF/BNF; 50 mg/kg each) and sampled at 2- and 6-day post-injection. Higher CYP1A protein expression in the liver and mRNA abundance in the head kidney confirmed AhR activation with BNF, but ANF did not abolish this response. BNF treatment did not significantly affect mRNA abundance of key steroidogenic enzymes (StAR protein, P450 side chain cleavage and 11beta-hydroxylase) in the head kidney of trout. However, BNF depressed the in vitro ACTH- or 8-br-cAMP-mediated cortisol production and this effect was also not modified by ANF. Plasma cortisol levels were significantly higher with BNF in the present study and this BNF-mediated cortisol response was completely abolished with ANF suggesting a role for AhR and/or CYP1A in the regulation of plasma cortisol concentration. However, despite elevated plasma cortisol levels, there was no significant effect of BNF on liver glucocorticoid receptor (GR) protein expression suggesting a lack of liver responsiveness to cortisol stimulation. Also, this was reflected in the absence of any change in plasma glucose concentration in the BNF group at 2 days, and a significant drop at 6 days compared to other treatments. Altogether, our results suggest that AhR activation disrupts interrenal corticosteroidogenesis and target tissue responsiveness to glucocorticoid stimulation in rainbow trout.
    [Abstract] [Full Text] [Related] [New Search]