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  • Title: Fluorescence polarization assay to quantify protein-protein interactions.
    Author: Park SH, Raines RT.
    Journal: Methods Mol Biol; 2004; 261():161-6. PubMed ID: 15064456.
    Abstract:
    A fluorescence polarization assay can be used to evaluate the strength of a protein-protein interaction. A green fluorescent protein variant is fused to one of the protein partners. The formation of a complex is then deduced from an increase in fluorescence polarization, and the equilibrium dissociation constant of the complex is determined in a homogeneous aqueous environment. The assay is demonstrated by using the interaction of the S-protein and S-peptide fragments of ribonuclease A as a case study.
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