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  • Title: Enzyme immunoassay and immunochemical characterization of pancreatic stone protein in human serum.
    Author: Katsuzaki T, Tatemichi N, Takeichi C, Hayakawa S, Hayakawa T, Shibata T, Nakae Y, Naruse S.
    Journal: J Clin Lab Anal; 1992; 6(3):143-7. PubMed ID: 1506981.
    Abstract:
    Monoclonal antibodies were raised against human pancreatic stone protein (PSP) and used for one-step enzyme immunoassay (EIA). PSP-S2-5 was employed as the standard in the assay. The assay's measurable range was 25-1,500 ng/ml and within run coefficient of variation was 3.7-6.4%. Analytical recovery of the assay was 101.5 +/- 5.65% (mean +/- SD). The results of experiments in which serum was fractionated by Mono S (cation exchange chromatography) suggested that most of immunoreactive material in human serum is PSP-S2-5. The EIA offers simple, rapid, and specific analysis of serum PSP level for clinical diagnosis.
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