These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Purification and pH stability characterization of a chymotrypsin inhibitor from Schizolobium parahyba seeds.
    Author: Teles RC, de Souza EM, Calderon Lde A, de Freitas SM.
    Journal: Phytochemistry; 2004 Apr; 65(7):793-9. PubMed ID: 15081278.
    Abstract:
    Schizolobium parahyba chymotrypsin inhibitor (SPCI) was completely purified as a single polypeptide chain with two disulfide bonds, by TCA precipitation and ion exchange chromatography. This purification method is faster and more efficient than that previously reported: SPCI is stable from pH 2 to 12 at 25 degrees C, and is highly specific for chymotrypsin at pH 7-12. It weakly inhibits elastase and has no significant inhibitory effect against trypsin and alpha-amylase. SPCI is a thermostable protein and resists thermolysin digestion up to 70 degrees C.
    [Abstract] [Full Text] [Related] [New Search]