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  • Title: [High-performance capillary electrophoresis for determining caffeic acid content in Fujie enema and Taraxacum mongolicum Hand.-Mazz].
    Author: Yan Y, Xu ZY, Chen ZL, Tan YF, Guo D.
    Journal: Di Yi Jun Yi Da Xue Xue Bao; 2004 Apr; 24(4):456-8. PubMed ID: 15090325.
    Abstract:
    OBJECTIVE: To determine caffeic acid content in Fujie enema and the crude traditional Chinese herbal drug Taraxacum mongolicum Hand.-Mazz. for controlling the quality of the enema at the levels of both the crude drugs and the final product. METHODS: Caffeic acid content in both the enema and the crude drug was determined at 313 nm Using high-performance capillary electrophoresis (HPCE), under the optimized conditions achieved with a fused-silica capillary tube (75 micromx0 cm) and 20 mmol/L borate running buffer (pH=9.18) at a constant voltage of 12 kV and a sampling time of 5 s at 25 degrees Celsius. RESULTS: The calibration curve displayed good linear relationship within caffeic acid concentration range of 10 to 100 microg/ml (r=0.999 2). The regression equation was Y=1002.45X-327.87, and the average recovery was more than 95%. CONCLUSION: HPCE is simple, rapid and sensitive in separation and determination of caffeic acid in Fujie enema and the crude drug of Taraxacum mongolicum Hand.-Mazz.
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