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  • Title: In vivo transgene expression using an adenoviral tetracycline-regulated system with neuron-specific enolase promoter.
    Author: Bhattacharjee AK, Ueyama T, Kondoh T, Hayashi S, Abouelfetouh A, Sakai N, Saito N, Kohmura E.
    Journal: Biochem Biophys Res Commun; 2004 May 14; 317(4):1144-8. PubMed ID: 15094388.
    Abstract:
    A recombinant adenoviral tetracycline-regulated system with neuron-specific enolase (NSE) promoter was injected stereotaxically into the striatum of rat brains. The efficiency of in vivo transfection was quantified by counting the number of green fluorescent protein (GFP)-positive cells at 3 days, 1 week, and 4 weeks after injection. NeuN immunohistochemistry demonstrated that expression of gammaPKC-GFP was dominant (20-99%) in neuron and expression of gammaPKC-GFP in neuron was significantly higher in pups than adult rats. These results indicate that tetracycline-inhibitable transcription factor (tTA) can drive tetracycline-responsive promoter (TetOp) under the control of NSE promoter, thereby efficiently and selectively expressing gammaPKC-GFP in neurons in vivo.
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