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  • Title: 16S to 23S rDNA spacer fragment length polymorphism of Aeromonas sp.
    Author: Laganowska M, Kaznowski A.
    Journal: Acta Microbiol Pol; 2003; 52(4):343-54. PubMed ID: 15095921.
    Abstract:
    We analyzed polymorphism of the PCR-amplified 16S-23S rDNA spacer of Aeromonas species. A total of 69 isolates representing 18 DNA hybridization groups were used in this study. The analysis of PCR products of 16S-23S rDNA spacers revealed patterns consisting of two to eight DNA fragments. The fragment sizes ranged from 730 to 1050 bp. DNA patterns revealed a considerable genetic diversity between species and within a species. When a procedure to eliminate heteroduplex formation was performed, the number of bands was reduced to 2-5. Nevertheless the homoduplex ISR (intergenic spacer region) patterns obtained were not useful for species distinguishing.
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