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  • Title: [Expression and identification of cecropin D gene cloned in Pichia pastoris yeast cells].
    Author: Fang S, Liu T, Deng P, Zhao S.
    Journal: Wei Sheng Yan Jiu; 2004 Jan; 33(1):81-5. PubMed ID: 15098486.
    Abstract:
    OBJECTIVE: Cecropin D gene was cloned into methanol nutritional vector-pPICZ alpha-A, linked with alpha-factor signal peptide. METHODS: Positive recombinant was gotten through PCR and EcoR I restriction enzyme. The positive recombinant vector was transformed into Pichia pastoris yeast strain GS115. RESULTS: The positive recombinant yeast velum was fermented and its expressive product was assayed by the method of anti-germ. CONCLUSION: Cecropin D gene was cloned into yeast cell and its expressive products has high activity of killing bacteria, Which could be developed into a new type of anti-bacterial medicine.
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