These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A supramolecular approach to protein labeling. A novel fluorescent bioassay for concanavalin a activity.
    Author: Rusin O, Král V, Escobedo JO, Strongin RM.
    Journal: Org Lett; 2004 Apr 29; 6(9):1373-6. PubMed ID: 15101745.
    Abstract:
    [reaction: see text] A new method for the bioassay of concanavalin A is based on the interaction of saccharide-substituted oligopyrrolic macrocycles with lectins. A general sensing mechanism involves the interaction of aggregated and primarily nonfluorescent labels with the target protein, label deaggregation, site-specific binding, and fluorescence signaling. Addition of saccharides to the fluorescent lectin-macrocycle complex leads to the release of the label and partial quenching of fluorescence due to reaggregation. Specificity of the protein-probe binding is discussed.
    [Abstract] [Full Text] [Related] [New Search]