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  • Title: Reversible immobilization of engineered molecules by Ni-NTA chelators.
    Author: Maly J, Di Meo C, De Francesco M, Masci A, Masojidek J, Sugiura M, Volpe A, Pilloton R.
    Journal: Bioelectrochemistry; 2004 Jun; 63(1-2):271-5. PubMed ID: 15110286.
    Abstract:
    Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)(6)-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor micro-array.
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