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  • Title: Determination and phylogenetic analysis of canine distemper virus in dogs with nervous symptoms in Turkey.
    Author: Ozkul A, Sancak AA, Güngör E, Burgu I.
    Journal: Acta Vet Hung; 2004; 52(1):125-32. PubMed ID: 15119793.
    Abstract:
    In the present study, canine distemper virus (CDV) was investigated in 20 dogs having nervous signs arousing the clinical suspicion of canine distemper (CD). A total of 13 animals (65%) were stray dogs and had no accurate record about the vaccination history. Clinical examinations revealed that the majority (85%) of the animals showed systemic form characterised by predominantly nervous symptoms accompanied by mild respiratory system signs whilst the remaining cases (15%) recorded mainly respiratory distress. CDV RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) only in 45% of the suspected cases. Phylogenetic analysis of partial nucleotide sequence of the P gene coding region revealed that the virus is closely related to European strains. Immune responses in 13 cases (65%), which were detected by dot-ELISA, indicated inefficient levels for neutralising functions against CDV. It was postulated that this response could have been mediated by either previous vaccination or mild infection with field strains.
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