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  • Title: [Comparative studies on proteins of cytoplasmic male-sterile wheat and its maintainer by 2D-PAGE in Triticum aestivum].
    Author: Fan BL, Wang ZY, Chen H, Peng YK.
    Journal: Shi Yan Sheng Wu Xue Bao; 2004 Feb; 37(1):45-9. PubMed ID: 15133897.
    Abstract:
    By using 2D-PAGE techniques, protein compositions have been studied at different developmental stage leaf (seedling, tillering, shooting and booting stages) and pollen mother cells (PMC) (meiotic, 1-nucleus and 2-3 nuclei stages) in male sterile wheat and its maintainer. The results indicated that a specific protein (33KD/pI6.3) identified at the shooting and booting stage leaves in male-sterile wheat, but this protein species has not been identified in the male fertile wheat. Four protein species, 53KD/PI5.5, 50KD/PI5.7, 48KD/PI5.6, 20KD/PI7.5 were detected at the 2-3 nuclei stages, which was thought key stage of pollen fertility lost in male-sterile wheat, and these protein species were not be seen in male-fertile wheat. According above experiment results, five protein species which detected in leaf and PMC might participate in fertility regulation and related to the male-sterility of male-sterile wheat.
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