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  • Title: Purification and characterization of inulinase from Aspergillus niger AF10 expressed in Pichia pastoris.
    Author: Zhang L, Zhao C, Zhu D, Ohta Y, Wang Y.
    Journal: Protein Expr Purif; 2004 Jun; 35(2):272-5. PubMed ID: 15135402.
    Abstract:
    The inuA1 gene encoding an exoinulinase from Aspergillus niger AF10 was expressed in Pichia pastoris, and the recombinant enzyme activity was 316U/ml in a 5L fermentor, with the inulinase protein accounting for 35% of the total protein of fermentation broth. The hydrolysis rate of inulin can reach 92%, with a 25U/g inulin enzyme addition, and 90% of fructose content after 6h. Glucose can significantly inhibit the enzymatic hydrolysis of inulin. This is the first report of glucose inhibition of inulinase-catalyzed hydrolysis.
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