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  • Title: Tuning the redox and enzymatic activity of glucose oxidase in layered organic films and its application in glucose biosensors.
    Author: Zhang W, Huang Y, Dai H, Wang X, Fan C, Li G.
    Journal: Anal Biochem; 2004 Jun 01; 329(1):85-90. PubMed ID: 15136170.
    Abstract:
    Glucose oxidase was embedded in organic films through a layer-by-layer approach, where the enzyme demonstrated significantly enhanced electron-transfer reactivity and finely tuned enzymatic activity. An unmediated, reagentless glucose biosensor was accordingly prepared with two polyethylenimine/glucose oxidase bilayers-modified pyrolytic graphite electrode. A calibration linear range of glucose was 0.5-8.9 mM with a detection limit of 50 microM and sensitivity of 0.76 microA mM(-1).
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