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  • Title: Interlaboratory reliability of microimmunofluorescence test for measurement of Chlamydia pneumoniae-specific immunoglobulin A and G antibody titers.
    Author: Littman AJ, Jackson LA, White E, Thornquist MD, Gaydos CA, Vaughan TL.
    Journal: Clin Diagn Lab Immunol; 2004 May; 11(3):615-7. PubMed ID: 15138190.
    Abstract:
    To evaluate the reliability of Chlamydia pneumoniae-specific immunoglobulin G (IgG) and IgA antibody titers as measured by the microimmunofluorescence (MIF) test, we compared results from 392 individuals using a standard MIF protocol at two academic laboratories. The kappas for dichotomous titers (>/=16 versus <16) were 0.39 for IgA and 0.53 for IgG. Measurement error likely attenuates C. pneumoniae-disease associations; the magnitude of attenuation can be estimated from results of studies such as this one.
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