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  • Title: Purification and characterization of thermostable xylanase and beta-xylosidase by the thermophilic bacterium Bacillus thermantarcticus.
    Author: Lama L, Calandrelli V, Gambacorta A, Nicolaus B.
    Journal: Res Microbiol; 2004 May; 155(4):283-9. PubMed ID: 15142626.
    Abstract:
    Bacillus thermantarcticus, a thermophilic bacterium isolated from Antarctic geothermal soil near the crater of Mount Melbourne, produced extracellular xylanase (1,4-beta-D-xylan xylanohydrolase; E.C. 3.2.1.8) and beta-xylosidase (1,4-beta-D-xylan xylohydrolase; E.C. 3.2.1.37). Each extracellular enzyme was separated by gel filtration with Sephacryl S-200 and further purified to homogeneity (119-fold for xylanase and 160-fold for beta-xylosidase). The optimum temperatures were 80 degrees C for xylanase at pH 5.6 and 70 degrees C for beta-xylosidase at pH 6.0. The isoelectric points and molecular masses were 4.8 and 45 kDa for xylanase and 4.2 and 150 kDa for beta-xylosidase, respectively. Xylanase was stable at 60 degrees C for 24 h, whereas it showed a half life at 70 degrees C of 24 h and at 80 degrees C for 50 min. beta-xylosidase activity did not decrease after 1 h at 60 degrees C. Km of xylanase for xylan was 1.6 mg/ml, Km of beta-xylosidase for p-nitrophenyl-beta-D-xylopyranoside was 0.5 mM and for o-nitrophenyl-beta-D-xylopyranoside was 1.28 mM. The action of two enzymes on xylan gave only xylose.
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