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  • Title: The N-terminal sequence directs import of mitochondrial alanine aminotransferase into mitochondria.
    Author: Metón I, Egea M, Fernández F, Eraso MC, Baanante IV.
    Journal: FEBS Lett; 2004 May 21; 566(1-3):251-4. PubMed ID: 15147904.
    Abstract:
    Herein, we report cloning and subcellular localization of two alanine aminotransferase (ALT) isozymes, cALT and mALT, from liver of gilthead sea bream (Sparus aurata). CHO cells transfected with constructs expressing cALT or mALT as C- or N-terminal fusion with the enhanced green fluorescent protein (EGFP) showed that cALT is cytosolic, whereas mALT localized to mitochondria. Fusion of EGFP to mALT N-terminus or removal of amino acids 1-83 of mALT avoided import into mitochondria, supporting evidence that the mALT N-terminus contains a mitochondrial targeting signal. The amino acid sequence of mALT is the first reported for a mitochondrial ALT in animals.
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