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  • Title: [Influence of the metabolite produced by Trichomonas vaginalis on human sperm motility in vitro].
    Author: Han Q, Liu J, Wang T, Xiao H, Fang Z.
    Journal: Zhonghua Nan Ke Xue; 2004 Apr; 10(4):272-4. PubMed ID: 15148923.
    Abstract:
    OBJECTIVE: To investigate the effects of the metabolite produced by Trichomonas vaginalis on human sperm motility in vitro. METHODS: Trichomonas vaginalis having been cultured, the culture solution containing metabolite was obtained by removing the protozoa, then diluted into 3 kinds of concentration. Sperm was obtained from 10 healthy fertile men by masturbation and prepared by swim-up technique to produce a spermatozoon solution of high motility. Every sperm sample was divided into 4 groups (A, B, C, D). Unused culture solution was added to Group A as control, and the other 3 groups (B, C, D) were respectively incubated with the above used culture solution at 3 kinds of concentration (1.2 x 10(9)/L, 6 x 10(8)/L, 1.2 x 10(8)/L). Measurements were carried out at 30 s, 1 h, 2 h, 4 h, 6 h by CASA. RESULTS: Sperm motility decreased in both Group B and C markedly, and the effects displayed a concentration- and time-dependent manner. CONCLUSION: The metabolite of Trichomonas vaginalis can reduce human sperm motility in vitro, and may be one of the causes of infertility.
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