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  • Title: G2 chromatid damage and repair kinetics in normal human fibroblast cells exposed to low- or high-LET radiation.
    Author: Kawata T, Ito H, Uno T, Saito M, Yamamoto S, Furusawa Y, Durante M, George K, Wu H, Cucinotta FA.
    Journal: Cytogenet Genome Res; 2004; 104(1-4):211-5. PubMed ID: 15162040.
    Abstract:
    Radiation-induced chromosome damage can be measured in interphase using the Premature Chromosome Condensation (PCC) technique. With the introduction of a new PCC technique using the potent phosphatase inhibitor calyculin-A, chromosomes can be condensed within five minutes, and it is now possible to examine the early damage induced by radiation. Using this method, it has been shown that high-LET radiation induces a higher frequency of chromatid breaks and a much higher frequency of isochromatid breaks than low-LET radiation. The kinetics of chromatid break rejoining consists of two exponential components representing a rapid and a slow time constant, which appears to be similar for low- and high-LET radiations. However, after high-LET radiation exposures, the rejoining process for isochromatid breaks influences the repair kinetics of chromatid-type breaks, and this plays an important role in the assessment of chromatid break rejoining in the G2 phase of the cell cycle.
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