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  • Title: A new ribonuclease from the black oyster mushroom Pleurotus ostreatus.
    Author: Wang HX, Ng TB.
    Journal: Peptides; 2004 Apr; 25(4):685-7. PubMed ID: 15165725.
    Abstract:
    A ribonuclease that is co-specific for poly C and poly U has been isolated from the fruiting bodies of the black oyster mushroom. The enzyme possesses a molecular mass of 14 kDa, and is unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel and CM-cellulose. A pH of 7 is required for the enzyme to exhibit maximal activity. The activity of the enzyme does not vary appreciably over the temperature range 30-60 degrees C, but drops when the temperature is reduced to 20 degrees C or raised to and above 70 degrees C. The ribonuclease does not exert any inhibitory activity toward HIV-1 reverse transcriptase.
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