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  • Title: Bile salt-independent retinyl ester hydrolase in the rat lacrimal gland.
    Author: Lopez Bernal D, Ubels JL.
    Journal: Int J Vitam Nutr Res; 1992; 62(2):186-90. PubMed ID: 1517043.
    Abstract:
    The lacrimal gland secretes retinol and synthesizes and stores retinyl esters. In this study, presence of retinyl ester hydrolase in rat lacrimal gland was investigated. A bile salt-independent retinyl ester hydrolase was identified in lacrimal gland homogenates and microsomes which were incubated in a tris-maleate buffer with retinyl palmitate as substrate. Retinol produced was measured by HPLC and activity expressed as pmoles retinol produced/mg protein/hr. In homogenates, the enzyme activity had a pH optimum at pH 6 and maximum specific activity of 57.7 pmoles/mg/hr. In microsomes, the activity was optimal at pH 7 and maximum specific activity was 1073 pmoles/mg/hr. In contrast, rat liver retinyl ester hydrolase had a pH optimum at pH 8 and a specific activity of 24.7 pmoles/mg/hr. These observations demonstrate that a complete cycle of retinyl ester metabolism is present in the lacrimal gland.
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