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  • Title: Purification of green fluorescent protein overexpressed by a mutant recombinant Escherichia coli.
    Author: Jain S, Teotia S, Gupta MN.
    Journal: Protein Expr Purif; 2004 Jul; 36(1):76-81. PubMed ID: 15177287.
    Abstract:
    Green fluorescent protein was purified from sonicated recombinant Escherichia coli and its mutant obtained after exposure to UV light. The latter overexpresses green fluorescent protein. The two-step procedure consisted of a two-phase aqueous extraction with PEG/salt and precipitation of the proteins from PEG phase by free Zn2+. The recoveries of green fluorescent protein were 73 and 83% in the cases of recombinant E. coli and its mutant, respectively. The corresponding fold purifications were 24 and 9, respectively. In both cases, the purified protein showed a single band on SDS-PAGE corresponding to 28 kDa.
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