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  • Title: Characterization of radioligand binding to a transmembrane receptor reconstituted into Lipobeads.
    Author: Park PS, Ng CC, Buck S, Wells JW, Cheng YL, Pennefather PS.
    Journal: FEBS Lett; 2004 Jun 04; 567(2-3):344-8. PubMed ID: 15178349.
    Abstract:
    Lipobeads are hydrogel beads surrounded by a lipid bilayer membrane and have been developed to act as a cell analogue. The FLAG-tagged M(2) muscarinic receptor was incorporated onto the surface of the Lipobead by incubating pre-Lipobeads with proteoliposomes containing the receptor. Receptors reconstituted onto the surface of the Lipobeads were functional in that they bound the antagonists quinuclidinylbenzilate and scopolamine with characteristic muscarinic affinities. This demonstrates the feasibility of using Lipobeads to study the binding properties of the M(2) muscarinic receptor and offers a promising approach to the study of transmembrane protein biology in general.
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