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  • Title: Detection of acid diffusion through bovine dentine after adhesive application.
    Author: Hiraishi N, Kitasako Y, Nikaido T, Foxton RM, Tagami J, Nomura S.
    Journal: Int Endod J; 2004 Jul; 37(7):455-62. PubMed ID: 15189434.
    Abstract:
    AIM: Acidic diffusion through bovine dentine was investigated by measuring pH changes on dentine surfaces after applying three adhesive systems. METHODOLOGY: Coronal incisor bovine dentine discs, 0.5 mm thick, were prepared from dentine close to the pulp chamber. A single-bottle adhesive system-Single Bond, a self-etching primer system-Clearfil SE Bond and an 'all-in-one' adhesive system-AQ Bond were used. The labial dentine surfaces were conditioned as follows: Single Bond groups: (SB-1) 35% phosphoric acid etchant was applied and left in place; (SB-2) the etchant was applied for 15 s and rinsed off for 10 s; (SB-3) application of adhesive agent and light curing following step SB-2; Clearfil SE Bond groups: (SE-1) SE primer was applied for 20 s and dried; (SE-2) application of adhesive agent and light curing following step SE-1; AQ Bond groups: (AQ-1) AQ Bond adhesive was applied for 20 s and dried, applied for additional 5 s and dried again; (AQ-2) light curing following step AQ-1. The pH change on the pulpal dentine surface was measured using a pH-imaging microscope. RESULTS: All the Single Bond groups revealed a lower pH on the pulpal surface (pH 6.25, 6.59 and 6.64 for SB-1, SB-2 and SB-3, respectively) compared with intact dentine. Clearfil SE Bond and AQ Bond groups showed no significant deference in pH value from intact dentine. CONCLUSIONS: Acid diffusion from phosphoric acid etching was observed when placed on 0.5 mm-thick dentine discs; however, there was only limited evidence of acid diffusion from SE primer and AQ Bond.
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