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  • Title: [Separation of high-quality sperm by PureSperm centrifugation applied to intrauterine insemination cycles].
    Author: Zhang Y, Qian Y, Feng T, Cai L, Liu J.
    Journal: Zhonghua Nan Ke Xue; 2004 May; 10(5):348-50. PubMed ID: 15190826.
    Abstract:
    OBJECTIVE: To separate high-quality sperm by PureSperm centrifugation applied to intrauterine insemination (IUI) cycles. METHODS: We compared the separate results after washing the semen with one-layer and two-layer PureSperm gradient centrifugation methods and two-layer Percoll gradient centrifugation method, and used the recovered high-quality sperm for IUI. RESULTS: The density of the sperm washed with one-layer PureSperm centrifugation method was significantly higher than that washed with two-layer Percoll and two-layer PureSperm centrifugation methods(P < 0.01), but there were no differences in all the results between the use of two-layer Percoll and two-layer PureSperm(P > 0.05). No significant differences in the motility, teratozoospermia and IUI results were found when the three methods were used for sperm preparation(P > 0.05). The percentage of morphologically normal sperm was markedly increased, and the non-sperm components such as leucocytes, epithelial cells and cellular fragments were significantly reduced after washed by the two methods. CONCLUSION: PureSperm centrifugation is a safe, efficient and easy method for separating high-quality sperm on intrauterine insemination cycles.
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