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  • Title: Cleaving of S-mandelonitrile catalyzed by S-hydroxynitrile lyase from Hevea brasiliensis--a kinetic investigation based on the rate curve method.
    Author: Yeow YL, Leong YK, Cheah MY, Dang HD, Law CK.
    Journal: J Biotechnol; 2004 Jul 01; 111(1):31-9. PubMed ID: 15196767.
    Abstract:
    The progress curves of conversion of racemic-mandelonitrile (R-MN) at different concentrations to benzaldehyde (BA) and hydrogen cyanide (HCN), catalyzed by S-hydroxynitrile lyase from Hevea brasiliensis, together with the enantiomeric excess curves of R-MN are converted into individual progress curves of the S- and R-enantiomers. They reveal that, under the prevailing experimental conditions, the non-enzymatic conversion is sufficiently slow compared to its enzyme-catalyzed counterpart, so that it can be ignored in a simplified analysis. Tikhonov regularization is then used to convert the progress curves of the S-enantiomer into reaction rate curves. These curves are used to determine the rate constants in the rate expression based on a three-step reversible ordered Uni-Bi reaction scheme that describes this enzyme-catalyzed reaction. The resulting rate constants are compared against published data. Some of the problems encountered and their solution are briefly discussed.
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