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  • Title: Identification of the heat shock protein 60 epitope involved in receptor binding on macrophages.
    Author: Habich C, Kempe K, Burkart V, Van Der Zee R, Lillicrap M, Gaston H, Kolb H.
    Journal: FEBS Lett; 2004 Jun 18; 568(1-3):65-9. PubMed ID: 15196922.
    Abstract:
    In the present study, we identified the human heat shock protein 60 (HSP60) epitope responsible for binding to macrophages. Studies using overlapping 15- and 20-mer peptides of the human HSP60 sequence to compete with binding of HSP60 to macrophages indicated that surface binding was accounted for by the region aa481-500. Deletion mutants of HSP60, lacking the N-terminal 137, 243 or 359 amino acids, strongly inhibited HSP60 binding to macrophages. Monoclonal antibodies addressing regions aa1-200, aa335-366 or aa383-447 did not block HSP60 binding. We conclude that a single C-terminal region, aa481-500, accounts for the binding of HSP60 to macrophages.
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