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  • Title: [Analysis of total proteins in the seed of almond (Prunus dulcis) by two-dimensional electrophoresis].
    Author: Li DD, He SH.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2004 Jul; 20(4):473-7. PubMed ID: 15207096.
    Abstract:
    AIM: To analyse the total proteins in the seeds of almond (Prunus dulcis), one of the popular ingestent allergens in China, by two-dimensional electrophoresis. METHODS: The total proteins of the seeds were extracted by trichloracetic acid (TCA) method, and then separated by isoelectric focusing as first dimension and SDS-PAGE as the second dimension. The spots of proteins were visualized by staining with Coomassie Brilliant Blue R-250. RESULTS: After analysis with software (ImageMaster 2D), 188 different proteins were detected. The isoelectric points (pI) for approximately 28% of total proteins were between 4.5-5.5, and the relative molecular mass (M(r)) of approximately 62% total proteins were between (20-25)x10(3). CONCLUSION: This was the first high-resolution, two-dimensional protein map of the seed of almond (Prunus dulcis) in China. Our finding has laid a solid foundation for further identification, characterization, gene cloning and standardization of allergenic proteins in the seed of almond (Prunus dulcis).
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