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  • Title: Modulation by dexamethasone of insulin binding and insulin receptor mRNA levels in U-937 human promonocytic cells.
    Author: Leal MA, Cabañas C, Rius C, Aller P, Calle C.
    Journal: Biochimie; 1992 Jun; 74(6):545-9. PubMed ID: 1520733.
    Abstract:
    Treatment with 5 x 10(-6) M dexamethasone stimulated insulin binding in human promonocytic U-937 cells. When curvilinear Scatchard plots were examined according to the one-site model, only changes in affinity, but not in receptor numbers, were observed. However, when the two-site model was applied, an increase in both the affinity and the number of the high affinity-low capacity sites was observed, with maximum values at 15 h. By contrast, the low affinity-high capacity sites did not undergo significant alterations. Northern blot assays revealed two insulin receptor-related mRNAs of approximately 11 and 7 kb in size. Dexamethasone increased the levels of these RNAs, following similar kinetics to those of high affinity receptor expression. This suggests that the 11 and 7 kb species carry information for high affinity insulin receptors, and that in U-937 cells the expression of this receptor subclass is primarily regulated at the mRNA level.
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