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  • Title: Overproduction and purification of lysyl-tRNA synthetase encoded by the herC gene of E coli.
    Author: Nakamura Y, Kawakami K.
    Journal: Biochimie; 1992 Jun; 74(6):581-4. PubMed ID: 1520738.
    Abstract:
    Lysyl-tRNA synthetases are synthesized from two distinct genes in E coli, lysS and lysU, but neither gene product has been purified distinctively by using overproducing systems. The lysS gene has been identified by a herC mutation which restores maintenance of the mutant ColE1 replicon. The herC gene product was overproduced by using a tac promoter fusion and purified to homogeneity. The purified HerC protein possesses a lysyl-tRNA synthetase activity as predicted by the sequence identity of herC to lysS. The procedure is useful for rapid mass-scale purification of lysyl-tRNA synthetase.
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