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  • Title: [Study on using the hen's egg test-chorioallantoic membrane as an alternative method of draize eye irritation test].
    Author: Liao Y, Wang X, Zhang L, Li G.
    Journal: Wei Sheng Yan Jiu; 2004 May; 33(3):279-83. PubMed ID: 15211792.
    Abstract:
    OBJECTIVE: To establish and study the hen's egg test-chorioallantoic membrane (HET-CAM) as an alternative method of Draize eye irritation test (Draize test). METHODS: 14 cosmetic ingredients were tested by the Hen's egg test-chorioallantoic membrane score (HET-CAM score) and the chorioallantoic membrane-trypan blue staining (CAM-TB) methods. RESULTS: Showed that compared with two kinds of scores in Draize test, i.e. Maximum average Draize total score (MAS) and score of 24 h after application (S24), the correlation coefficient between HET-CAM score and MAS or S24 was 0.847 or 0.779, while that between CAB-TB and MAS or S24 was 0.862 or 0.831 respectively. The results also showed that CAM-TB had a higher correlation with Draize test than HET-CAM score, partly because it is objective and quantitative. Also, the results showed that HET-CAM score had the greatest correlation with conjunctivae score of the three components, and so did the CAM-TB with corneal score. CONCLUSION: It is suggested that the two types of HET-CAM can be used in a combined manner as an effective alternative method to Draize test.
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