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  • Title: Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea.
    Author: Shiba H, Park JI, Suzuki G, Matsushita M, Nou IS, Isogai A, Takayama S, Watanabe M.
    Journal: Genes Genet Syst; 2004 Apr; 79(2):87-93. PubMed ID: 15215674.
    Abstract:
    Self-incompatibility (SI) discriminating self and non-self pollen is regulated by S-locus genes in Brassica. In most S haplotypes, a set of three highly polymorphic genes, SLG, SRK, and SP11, is located at the S-locus region. In the present study, we found duplicated SP11 genes, S15-SP11a, S15-SP11b, and S15-SP11b', in the self-incompatible S15 haplotype of B. oleracea. RNA gel blot and reverse transcription polymerase chain reaction (RT-PCR) analyses showed that two different sizes of SP11 transcripts were specifically detected in anther tissues: a 0.65-kb transcript corresponded to S15-SP11a (an exon-1 region of S15-SP11b was also co-transcribed in some cases), and a 1.4-kb transcript contained the duplicated three genes, S15-SP11a, S15-SP11b, and an exon-1 region of S15-SP11b', all three of which were connected to intergenic spacer regions.
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