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  • Title: Detection of circulating antigens in experimental anisakiasis by two-site enzyme-linked immunosorbent assay.
    Author: Campos M, Martín L, Díaz V, Mañas I, Morales B, Lozano J.
    Journal: Parasitol Res; 2004 Aug; 93(6):433-8. PubMed ID: 15221461.
    Abstract:
    A two-site enzyme-linked immunosorbent assay (ELISA) based on the avidin-biotin system was developed to study circulating antigens in the sera of rats orally or intraperitoneally infected with L3 larvae of Anisakis simplex s.l. Somatic and excretory-secretory antigens were detected from 24 h post-infection in all infected rats. A direct relationship between the number of larvae inoculated and the concentration of antigens in the sera was demonstrated; however, the amount of antigen decreased with the course of the infection. Comparative analysis revealed that intraperitoneal inoculation is most appropriate for detecting excretory-secretory antigens in serum. The minimum concentrations of A. simplex antigen detectable were approximately 2.5 microg/ml for somatic and excretory-secretory antigens. This antigen detection assay may have application in the diagnosis of anisakiasis.
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