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  • Title: Rapid discrimination between strains of beta haemolytic streptococci by intact cell mass spectrometry.
    Author: Kumar MP, Vairamani M, Raju RP, Lobo C, Anbumani N, Kumar CP, Menon T, Shanmugasundaram S.
    Journal: Indian J Med Res; 2004 Jun; 119(6):283-8. PubMed ID: 15243166.
    Abstract:
    BACKGROUND & OBJECTIVES: Beta haemolytic streptococci belonging to Lancefield group A, B, C and G cause a wide spectrum of clinical diseases. Hence there is a need for rapid and accurate typing of these strains. The present study was undertaken to evaluate the use of intact cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for rapid discrimination between strains of beta haemolytic streptococci. METHODS: Colonies of beta haemolytic streptococci were emulsified with chemical matrix on the sample slide, dried and analyzed by MALDI-TOF-MS. RESULTS: The reproducibility of results for all groups of beta haemolytic streptococci was good and spectra obtained for Lancefield group A, C and G streptococci showed discrimination between the groups on visual comparison. A finer difference in spectrum was observed among group A streptococci isolated from different locations at different periods of time. INTERPRETATION & CONCLUSION: MALDI-TOF-MS may be a potential tool in discriminating between strains of beta haemolytic streptococci, and also in the characterisation of untypable strains of group A streptococci.
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