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  • Title: [Design of a DNA-matrix with a specific structure for synthesizing RNA using the polymerase chain reaction].
    Author: Smelkova NV, Elov AA, Shabarova ZA.
    Journal: Bioorg Khim; 1992 Jan; 18(1):78-84. PubMed ID: 1524586.
    Abstract:
    Combination of chemical DNA synthesis and polymerase chain reactions has been used for obtaining DNA templates of preset structure coding for target mRNAs. The obtained DNA were 136 to 246 bp in length, contained promoters for RNA polymerases of phage T7 or E. coli and coded for 136-membered mRNA.
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