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  • Title: Analysis of sugar phosphates in plants by ion chromatography on a titanium dioxide column with pulsed amperometric detection.
    Author: Sekiguchi Y, Mitsuhashi N, Inoue Y, Yagisawa H, Mimura T.
    Journal: J Chromatogr A; 2004 Jun 11; 1039(1-2):71-6. PubMed ID: 15250405.
    Abstract:
    This paper describes the development of a practical method for the analysis of sugar phosphates from the model higher plant Arabidopsis thaliana by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The extraction method of sugar phosphates from higher plants was first optimized for HPAEC-PAD analysis. In order to improve the resolution in HPAEC-PAD, a column packed with titanium dioxide resin was used. The titanium dioxide column was used as a trap-column for sugar phosphates and nucleotides, for the removal of sample matrices. Sample pretreatment was achieved in-line and automatically using a six-port valve placed after the injection valve.
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