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  • Title: The presence of a high-Km hexokinase activity in dog, but not in boar, sperm.
    Author: Fernández-Novell JM, Ballester J, Medrano A, Otaegui PJ, Rigau T, Guinovart JJ, Rodríguez-Gil JE.
    Journal: FEBS Lett; 2004 Jul 16; 570(1-3):211-6. PubMed ID: 15251466.
    Abstract:
    The presence of a high-Km hexokinase activity was tested in both dog and boar spermatozoa. Hexokinase kinetics from dog extracts showed the presence of a specific activity (dog-sperm glucokinase-like protein, DSGLP), in the range of glucose concentrations of 4-10 mM, whereas boar sperm did not show any DSGLP activity. Furthermore, dog-sperm cells, but not those of boar, showed the presence of a protein which specifically reacted against a rat-liver anti-glucokinase antibody. This protein also had a molecular weight equal to that observed in rat-liver extracts, suggesting a close similarity between both the proteins. This glucokinase-like protein was distributed in the peri- and post-acrosomal zones of the head, and the midpiece and principal piece of tail of dog spermatozoa. These results indicate that dog spermatozoa have functional high-Km hexokinase activity, which could contribute to a very fine regulation of their hexose metabolism. This strict regulation could ultimately be very important in optimizing dog-sperm function along its life-time.
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