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  • Title: Isolation of nucleic acid binding proteins: an approach for isolation of cell surface, nucleic acid binding proteins.
    Author: Chelobanov BP, Laktionov PP, Kharkova MV, Rykova EY, Vlassov VV.
    Journal: Ann N Y Acad Sci; 2004 Jun; 1022():239-43. PubMed ID: 15251967.
    Abstract:
    An approach for isolation of cell surface, nucleic acid binding proteins is described. This approach relies on affinity modification of the proteins of living cells with reactive oligonucleotides bearing a haptenic group. Covalently modified proteins were isolated by hapten-specific affinity chromatography with subsequent SDS-PAGE. Isolated 68-kDa proteins responsible for the binding of oligonucleotides were MS/MS sequenced and identified as keratin K1, keratin K10, keratin K2e, and albumin.
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