These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Possible role of interleukin-1 in the regulation of bovine corpus luteum throughout the luteal phase.
    Author: Nishimura R, Bowolaksono A, Acosta TJ, Murakami S, Piotrowska K, Skarzynski DJ, Okuda K.
    Journal: Biol Reprod; 2004 Nov; 71(5):1688-93. PubMed ID: 15253926.
    Abstract:
    Interleukin-1 (IL-1) is one of the principal cytokines that participate in local regulation of many reproductive functions. The present study was undertaken to determine whether mRNAs for IL-1alpha, IL-1beta, and IL-1 type I receptor (IL-1R) are expressed in bovine corpora lutea (CL), and whether luteal cells respond to treatment with IL-1alpha and IL-1beta during the luteal phase. Bovine CL were classified into five stages (early, Days 2-3; developing, Days 5-6; mid, Days 8-12; late, Days 15-17; and regressed, Days 19-21). IL-1alpha, IL-1beta, and IL-1R mRNAs were detected by reverse transcription-polymerase chain reaction (PCR) in all luteal stages examined. Densitometric analysis of PCR products revealed increases of the mRNA of IL-1alpha and IL-1R in the CL of the regressed stage (P < 0.05). There was less mRNA for IL-1beta in the regressed stage than in the developing and mid stages (P < 0.05). When developing, mid, and late luteal cells were treated with IL-1alpha (1-30 ng/ml) or IL-1beta (1-30 ng/ml) for 24 h, IL-1alpha and IL-1beta dose-dependently increased prostaglandin (PG) F(2alpha) and PGE(2) production by the luteal cells of all stages (P < 0.05), indicating the presence of functional IL-1R in bovine CL. However, progesterone synthesis was not affected by either IL-1alpha or IL-1beta treatment. Stimulation with IL-1alpha and IL-1beta decreased the PGE(2):PGF(2alpha) ratio in the developing stage (P < 0.05), whereas it increased the ratio in the mid stage (P < 0.05). In the late stage, the ratio of IL-1beta-treated cells was greater than that of IL-1alpha-treated cells (P < 0.05). Overall results indicate that genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in the bovine CL throughout the luteal phase, and suggest that IL-1alpha and IL-1beta have different roles as local modulators to regulate PGF(2alpha) and PGE(2) production during the luteal phase.
    [Abstract] [Full Text] [Related] [New Search]