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  • Title: Boar seminal zinc-precipitable protein and the haemagglutinin.
    Author: Boursnell JC, Noble EA, Andrews MG.
    Journal: J Reprod Fertil; 1975 Dec; 45(3):415-20. PubMed ID: 1526.
    Abstract:
    About 30% of boar seminal plasma nitrogen is maximally precipitated at room temperature by 6 to 10 mM zinc in citrate solution at pH 8. A rise in the total nitrogen precipitated by 1 to 6 mM zinc is accompanied by a fall in the haemagglutinin titre of the supernatant fluid. At 6 mM zinc addition, 95% of the haemagglutinin is precipitated, but much of this is recoverable by re-solution of the zinc precipitate. Protein profile studies by gel-filtration chromatography of the zinc precipitate solution reveals a mixture of proteins, some of which are not by themselves zinc-precipitable.
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